RRC ID 81927
Author Matsuda K, Kariya R, Maeda K, Okada S.
Title Evaluating the Use of Sacran, a Polysaccharide Isolated from Aphanothece sacrum, as a Possible Microbicide for Preventing HIV-1 Infection.
Journal Viruses
Abstract Since combination antiretroviral therapy (cART) was introduced to treat human immunodeficiency virus type-1 (HIV-1)/acquired immunodeficiency syndrome (AIDS), the AIDS mortality rate has markedly decreased, and convalescence in individuals with HIV has improved drastically. However, sexual transmission has made HIV-1 a global epidemic. Sacran is a megamolecular polysaccharide extracted from cyanobacterium Aphanothece sacrum that exhibits numerous desirable characteristics for transdermic applications, such as safety as a biomaterial, a high moisture retention effect, the ability to form a film and hydrogel, and an anti-inflammatory effect. In this study, we evaluated the anti-HIV-1 effects in sacran as a barrier to HIV-1 transmission. Sacran inhibited HIV-1 infection and envelope-dependent cell-to-cell fusion. Moreover, we used a Transwell assay to confirm that sacran inhibited viral diffusion and captured viruses. The synergistic effects of sacran and other anti-HIV infection drugs were also evaluated. HIV-1 infections can be reduced through the synergistic effects of sacran and anti-HIV-1 drugs. Our study suggests using sacran gel to provide protection against HIV-1 transmission.
Volume 16(9)
Published 2024-9-23
DOI 10.3390/v16091501
PII v16091501
PMID 39339979
PMC PMC11437396
MeSH Anti-HIV Agents / pharmacology Anti-Infective Agents / pharmacology Cell Line Cyanobacteria / chemistry HIV Infections* / prevention & control HIV Infections* / virology HIV-1* / drug effects Humans Polysaccharides / pharmacology Polysaccharides, Bacterial / pharmacology Virus Internalization / drug effects
Resource
Human and Animal Cells MOLT-4(RCB0206)