論文 - 詳細
| RRC ID | 81935 |
|---|---|
| 著者 | Tsuji T, Hasegawa J, Sasaki T, Fujimoto T. |
| タイトル | Definition of phosphatidylinositol 4,5-bisphosphate distribution by freeze-fracture replica labeling. |
| ジャーナル | J Cell Biol |
| Abstract |
Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] is a phospholipid essential for plasma membrane functions, but its two-dimensional distribution is not clear. Here, we compared the result of sodium dodecyl sulfate-treated freeze-fracture replica labeling (SDS-FRL) of quick-frozen cells with the actual PtdIns(4,5)P2 content and the results obtained by fluorescence biosensor and by labeling of chemically-fixed membranes. In yeast, enrichment of PtdIns(4,5)P2 in the membrane compartment of Can1 (MCC)/eisosome, especially in the curved MCC/eisosome, was evident by SDS-FRL, but not by fluorescence biosensor, GFP-PLC1δ-PH. PtdIns(4,5)P2 remaining after acute ATP depletion and in the stationary phase, 30.0% and 56.6% of the control level, respectively, was not detectable by fluorescence biosensor, whereas the label intensity by SDS-FRL reflected the PtdIns(4,5)P2 amount. In PC12 cells, PtdIns(4,5)P2 was observed in a punctate pattern in the formaldehyde-fixed plasma membrane, whereas it was distributed randomly by SDS-FRL and showed clustering after formaldehyde fixation. The results indicate that the distribution of PtdIns(4,5)P2 can be defined most reliably by SDS-FRL of quick-frozen cells. |
| 巻・号 | 224(1) |
| 公開日 | 2025-1-6 |
| DOI | 10.1083/jcb.202311067 |
| PII | 277060 |
| PMID | 39495319 |
| PMC | PMC11535894 |
| MeSH | Adenosine Triphosphate / metabolism Animals Biosensing Techniques / methods Cell Membrane* / metabolism Freeze Fracturing* PC12 Cells Phosphatidylinositol 4,5-Diphosphate* / metabolism Rats Saccharomyces cerevisiae* / metabolism Sodium Dodecyl Sulfate / pharmacology Staining and Labeling / methods |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |