RRC ID 81955
Author Yuasa H, Miyazaki K, Kido T, Nishino K, Shiotsu M, Fukuwatari T, Suka M, Nagao M, Kambe T.
Title Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo.
Journal FASEB J
Abstract Extracellular hydrolysis of the phosphate esters of B vitamins (B1, B2, and B6) is crucial for their cellular uptake and metabolism. Although a few zinc-dependent enzymes have been implicated in these processes, their exact mechanisms of action remain largely unknown. This study investigated the potential involvement of phosphate group hydrolyzing enzymes in the hydrolysis of B vitamin phosphate esters. We evaluated enzyme activity in membrane lysates prepared from cells transiently transfected with these enzymes or those endogenously expressing them. Specifically, we investigated how zinc deficiency affects the rate of hydrolysis of B vitamin phosphate esters in cellular lysates. Assessment of the activities of zinc-dependent ectoenzymes in the lysates prepared from cells cultured in zinc-deficient conditions and in the serum of rats fed zinc-deficient diets revealed that zinc deficiency reduced the extracellular hydrolysis activity of B vitamin phosphate esters. Furthermore, our findings explain the similarities between several symptoms of B vitamin and zinc deficiencies. Collectively, this study provides novel insights into the diverse symptoms of zinc deficiency and could guide the development of appropriate clinical strategies.
Volume 38(18)
Pages e70025
Published 2024-9-30
DOI 10.1096/fj.202401207R
PMID 39279493
MeSH Animals Esters* / metabolism Humans Hydrolysis Male Phosphates / deficiency Phosphates / metabolism Rats Rats, Wistar Vitamin B 6 / metabolism Vitamin B Complex / metabolism Zinc* / deficiency Zinc* / metabolism
Resource
DNA material IRAK034P02 (HGY013962)