論文 - 詳細
| RRC ID | 81957 |
|---|---|
| 著者 | Matsumoto R, Ogata K, Takahashi D, Kinashi Y, Yamada T, Morita R, Tanaka K, Hattori K, Endo M, Fujimura Y, Sasaki N, Ohno H, Ishihama Y, Kimura S, Hase K. |
| タイトル | AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine. |
| ジャーナル | Cell Mol Life Sci |
| Abstract |
Intraepithelial lymphocytes (IELs) reside in the epithelial layer and protect against foreign pathogens, maintaining the epithelial barrier function in the intestine. Interactions between IEL and epithelial cells are required for IELs to function effectively; however, the underlying molecular machinery remains to be elucidated. In this study, we found that intestinal epithelium-specific deficiency of the clathrin adaptor protein (AP)-1B, which regulates basolateral protein sorting, led to a massive reduction in IELs. Quantitative proteomics demonstrated that dozens of proteins, including known IEL-interacting proteins (E-cadherin, butyrophilin-like 2, and plexin B2), were decreased in the basolateral membrane of AP-1B-deficient epithelial cells. Among these proteins, CD166 interacted with CD6 on the surface of induced IEL. CD166 knockdown, using shRNA in intestinal organoid cultures, significantly inhibited IEL recruitment to the epithelial layer. These findings highlight the essential role of AP-1B-mediated basolateral sorting in IEL maintenance and survival within the epithelial layer. This study reveals a novel function of AP-1B in the intestinal immune system. |
| 巻・号 | 81(1) |
| ページ | 425 |
| 公開日 | 2024-10-5 |
| DOI | 10.1007/s00018-024-05455-1 |
| PII | 10.1007/s00018-024-05455-1 |
| PMID | 39369131 |
| PMC | PMC11455912 |
| MeSH | Adaptor Protein Complex 1* / genetics Adaptor Protein Complex 1* / metabolism Animals Antigens, CD* / genetics Antigens, CD* / metabolism Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / metabolism Cell Communication Epithelial Cells* / immunology Epithelial Cells* / metabolism Intestinal Mucosa* / cytology Intestinal Mucosa* / immunology Intestinal Mucosa* / metabolism Intestines / cytology Intestines / immunology Intraepithelial Lymphocytes* / immunology Intraepithelial Lymphocytes* / metabolism Mice Mice, Inbred C57BL |
| IF | 6.496 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pENTR4-H1 (RDB04395) CSII-EF-mRFP1 (RDB06387) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |