論文 - 詳細
| RRC ID | 82016 |
|---|---|
| 著者 | Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA. |
| タイトル | eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. |
| ジャーナル | EMBO J |
| Abstract |
eIF3, whose subunits are frequently overexpressed in cancer, regulates mRNA translation from initiation to termination, but mRNA-selective functions of individual subunits remain poorly defined. Using multiomic profiling upon acute depletion of eIF3 subunits, we observed that while eIF3a, b, e, and f markedly differed in their impact on eIF3 holo-complex formation and translation, they were each required for cancer cell proliferation and tumor growth. Remarkably, eIF3k showed the opposite pattern with depletion promoting global translation, cell proliferation, tumor growth, and stress resistance through repressing the synthesis of ribosomal proteins, especially RPS15A. Whereas ectopic expression of RPS15A mimicked the anabolic effects of eIF3k depletion, disruption of eIF3 binding to the 5'-UTR of RSP15A mRNA negated them. eIF3k and eIF3l are selectively downregulated in response to endoplasmic reticulum and oxidative stress. Supported by mathematical modeling, our data uncover eIF3k-l as a mRNA-specific module which, through controlling RPS15A translation, serves as a rheostat of ribosome content, possibly to secure spare translational capacity that can be mobilized during stress. |
| 巻・号 | 42(12) |
| ページ | e112362 |
| 公開日 | 2023-6-15 |
| DOI | 10.15252/embj.2022112362 |
| PMID | 37155573 |
| PMC | PMC10267700 |
| MeSH | Eukaryotic Initiation Factor-3* / genetics Eukaryotic Initiation Factor-3* / metabolism Humans Neoplasms* / genetics Neoplasms* / metabolism Protein Biosynthesis RNA, Messenger / genetics RNA, Messenger / metabolism Ribosomal Proteins / genetics Ribosomal Proteins / metabolism Ribosomes / genetics Ribosomes / metabolism |
| IF | 9.889 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCT116 CMV-OsTIR1(RCB4662) |