| RRC ID |
82033
|
| Author |
Kunimatsu R, Hiraki T, Rikitake K, Nakajima K, Putranti NAR, Abe T, Ando K, Nakatani A, Sakata S, Tanimoto K.
|
| Title |
Effects of Human Deciduous Dental Pulp-Derived Mesenchymal Stem Cell-Derived Conditioned Medium on the Metabolism of HUVECs, Osteoblasts, and BMSCs.
|
| Journal |
Cells
|
| Abstract |
In this study, we assessed the effects of human deciduous dental pulp-derived mesenchymal stem cell-derived conditioned medium (SHED-CM) on the properties of various cell types. The effects of vascular endothelial growth factor (VEGF) in SHED-CM on the luminal architecture, proliferative ability, and angiogenic potential of human umbilical vein endothelial cells (HUVECs) were determined. We also investigated the effects of SHED-CM on the proliferation of human-bone-marrow mesenchymal stem cells (hBMSCs) and mouse calvarial osteoblastic cells (MC3T3-E1) as well as the expression of ALP, OCN, and RUNX2. The protein levels of ALP were examined using Western blot analysis. VEGF blockade in SHED-CM suppressed the proliferative ability and angiogenic potential of HUVECs, indicating that VEGF in SHED-CM contributes to angiogenesis. The culturing of hBMSCs and MC3T3-E1 cells with SHED-CM accelerated cell growth and enhanced mRNA expression of bone differentiation markers. The addition of SHED-CM enhanced ALP protein expression in hBMSCs and MT3T3-E1 cells compared with that of the 0% FBS group. Furthermore, SHED-CM promoted the metabolism of HUVECs, MC3T3-E1 cells, and hBMSCs. These findings indicate the potential benefits of SHED-CM in bone tissue regeneration.
|
| Volume |
11(20)
|
| Published |
2022-10-14
|
| DOI |
10.3390/cells11203222
|
| PII |
cells11203222
|
| PMID |
36291089
|
| PMC |
PMC9600042
|
| MeSH |
Animals
Core Binding Factor Alpha 1 Subunit / metabolism
Culture Media, Conditioned* / metabolism
Culture Media, Conditioned* / pharmacology
Dental Pulp* / cytology
Human Umbilical Vein Endothelial Cells* / drug effects
Human Umbilical Vein Endothelial Cells* / metabolism
Humans
Mesenchymal Stem Cells* / metabolism
Mice
Osteoblasts* / drug effects
Osteoblasts* / metabolism
RNA, Messenger / metabolism
Tooth, Deciduous* / cytology
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
4.366
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
