RRC ID 82060
Author Lin CY, Ching YY, Wu SF, Lee YK, Fan HC, Su LY, Tsai SY, Chen YC, Shen CI, Su HL.
Title Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells.
Journal Cell Transplant
Abstract Cell expansion of human pluripotent stem cells (hPSCs) commonly depends on Matrigel as a coating matrix on two-dimensional (2D) culture plates and 3D microcarriers. However, the xenogenic Matrigel requires sophisticated quality-assurance processes to meet clinical requirements. In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process. The pluripotent stemness of the expanded cells is stably sustained for more than 10 passages, featured with high pluripotent marker expressions, normal karyotyping, and differentiating capacity for three germ layers. The expression levels of some integrins are reduced, compared with those of the hPSCs on Matrigel. This medium also successfully supports the clonal expansion and induced pluripotent stem cell establishment from mitochondrial-defective MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes) patient's peripheral blood mononuclear cells. This innovative hPSC medium provides a straightforward scale-up process for producing clinical-orientated hPSCs by excluding the conventional coating procedure.
Volume 32
Pages 9636897231198172
Published 2023-1-1
DOI 10.1177/09636897231198172
PMID 37698258
PMC PMC10498698
MeSH Cell Culture Techniques / methods Cell Differentiation Humans Induced Pluripotent Stem Cells* Leukocytes, Mononuclear Pluripotent Stem Cells* / metabolism
Resource
Human and Animal Cells 253G1(HPS0002)