RRC ID 82062
Author Ishigaki M, Hitomi H, Ozaki Y, Nishiyama A.
Title Exposing intracellular molecular changes during the differentiation of human-induced pluripotent stem cells into erythropoietin-producing cells using Raman spectroscopy and imaging.
Journal Sci Rep
Abstract The objective of this study was to explore intracellular molecular changes during the differentiation of human-induced pluripotent stem cells (iPSCs) into erythropoietin (EPO)-producing cells using Raman spectroscopy and imaging. Raman imaging data of fixed cells at four stages of cell differentiation were analyzed by a partial least squares (PLS) regression model, and the variations in the intracellular molecular compositions with cell differentiation were investigated. As a result, three biomarkers characterizing the cell phases were identified: dimethyl sulfoxide (DMSO), fatty acids with a low grade of unsaturation, and glycoproteins. The uptake of DMSO by EPO-producing cells, which was added into a culture medium as an inducer for cell differentiation, was detected, and the increase in unsaturated fatty acid concentrations was revealed that lipid metabolism changed over the course of cell differentiation. The decrease in the glycoprotein concentration after the cell phase during which iPSCs differentiated into EPO-producing cells was also made clear. Raman imaging successfully visualized chemical images of these three biomarkers in two dimensions, where the biomarker concentrations independently varied during cell differentiation. These results demonstrated the application potential of the proposed method to regenerative medicine for monitoring cell differentiation and discriminating cell maturation in situ at the molecular level.
Volume 12(1)
Pages 20454
Published 2022-11-28
DOI 10.1038/s41598-022-24725-1
PII 10.1038/s41598-022-24725-1
PMID 36443362
PMC PMC9705388
MeSH Cell Differentiation Dimethyl Sulfoxide Erythropoietin* Humans Induced Pluripotent Stem Cells* Spectrum Analysis, Raman
Resource
Human and Animal Cells 253G1(HPS0002)