| RRC ID |
82088
|
| 著者 |
Mubuchi A, Takechi M, Nishio S, Matsuda T, Itoh Y, Sato C, Kitajima K, Kitagawa H, Miyata S.
|
| タイトル |
Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons.
|
| ジャーナル |
Elife
|
| Abstract |
Radial neuronal migration is a key neurodevelopmental event for proper cortical laminar organization. The multipolar-to-bipolar transition, a critical step in establishing neuronal polarity during radial migration, occurs in the subplate/intermediate zone (SP/IZ), a distinct region of the embryonic cerebral cortex. It has been known that the extracellular matrix (ECM) molecules are enriched in the SP/IZ. However, the molecular constitution and functions of the ECM formed in this region remain poorly understood. Here, we identified neurocan (NCAN) as a major chondroitin sulfate proteoglycan in the mouse SP/IZ. NCAN binds to both radial glial-cell-derived tenascin-C (TNC) and hyaluronan (HA), a large linear polysaccharide, forming a ternary complex of NCAN, TNC, and HA in the SP/IZ. Developing cortical neurons make contact with the ternary complex during migration. The enzymatic or genetic disruption of the ternary complex impairs radial migration by suppressing the multipolar-to-bipolar transition. Furthermore, both TNC and NCAN promoted the morphological maturation of cortical neurons in vitro. The present results provide evidence for the cooperative role of neuron- and radial glial-cell-derived ECM molecules in cortical development.
|
| 巻・号 |
12
|
| 公開日 |
2024-3-21
|
| DOI |
10.7554/eLife.92342
|
| PII |
92342
|
| PMID |
38512724
|
| PMC |
PMC10957175
|
| MeSH |
Animals
Cell Movement / physiology
Cerebral Cortex / metabolism
Chondroitin Sulfate Proteoglycans / metabolism
Extracellular Matrix* / metabolism
Mice
Neurons* / physiology
|
| IF |
7.08
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637)
293T(RCB2202) |
