論文 - 詳細
| RRC ID | 82099 |
|---|---|
| 著者 | Ichinose M, Kawabata M, Akaiwa Y, Shimajiri Y, Nakamura I, Tamai T, Nakamura T, Yagi Y, Gutmann B. |
| タイトル | U-to-C RNA editing by synthetic PPR-DYW proteins in bacteria and human culture cells. |
| ジャーナル | Commun Biol |
| Abstract |
Programmable RNA editing offers significant therapeutic potential for a wide range of genetic diseases. Currently, several deaminase enzymes, including ADAR and APOBEC, can perform programmable adenosine-to-inosine or cytidine-to-uridine RNA correction. However, enzymes to perform guanosine-to-adenosine and uridine-to-cytidine (U-to-C) editing are still lacking to complete the set of transition reactions. It is believed that the DYW:KP proteins, specific to seedless plants, catalyze the U-to-C reactions in mitochondria and chloroplasts. In this study, we designed seven DYW:KP domains based on consensus sequences and fused them to a designer RNA-binding pentatricopeptide repeat (PPR) domain. We show that three of these PPR-DYW:KP proteins edit targeted uridine to cytidine in bacteria and human cells. In addition, we show that these proteins have a 5' but not apparent 3' preference for neighboring nucleotides. Our results establish the DYW:KP aminase domain as a potential candidate for the development of a U-to-C editing tool in human cells. |
| 巻・号 | 5(1) |
| ページ | 968 |
| 公開日 | 2022-9-15 |
| DOI | 10.1038/s42003-022-03927-3 |
| PII | 10.1038/s42003-022-03927-3 |
| PMID | 36109586 |
| PMC | PMC9478123 |
| MeSH | Adenosine / metabolism Bacteria / genetics Bacteria / metabolism Cytidine* / genetics Cytidine* / metabolism Guanosine / metabolism Humans Inosine Nucleotides / metabolism Plant Proteins / genetics RNA / metabolism RNA Editing* Uridine / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |