Reference - Detail
| RRC ID | 82144 |
|---|---|
| Author | Peng Y, Iwasaki K, Taguchi Y, Ishikawa I, Umeda M. |
| Title | Mesenchymal stem cell-derived protein extract induces periodontal regeneration. |
| Journal | Cytotherapy |
| Abstract |
BACKGROUND:Periodontal disease is characterized by chronic inflammation and destruction of supporting periodontal tissues, ultimately leading to tooth loss. In recent years, "cell-free treatment" without stem cell transplantation has attracted considerable attention for tissue regeneration. This study investigated the effects of extracts of mesenchymal stem cells (MSC-extract) and their protein components (MSC-protein) on the proliferation and migration of periodontal ligament (PDL) cells and whether MSC-protein can induce periodontal regeneration. METHODS:MSC-extract and MSC-protein were obtained by subjecting mesenchymal stem cells (MSCs) to freeze-thaw cycles and acetone precipitation. Cell proliferation was examined using a WST-8 assay and Ki67 immunostaining, and cell migration was examined using Boyden chambers. The MSC-protein content was analyzed using liquid chromatography-mass spectrometry, protein arrays, and enzyme-linked immunosorbent assays (ELISAs). Gene expression in MSC-protein-treated PDL cells was examined using RNA-sequencing and Gene Ontology analyses. The regenerative potential of MSC-protein was examined using micro-computer tomography (CT) and histological analyses after transplantation into a rat periodontal defect model. RESULTS:MSC-extract and MSC-protein promoted the proliferation and migration of PDL cells. Protein array and ELISA revealed that MSC-protein contained high concentrations of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Exogenous bFGF promoted the proliferation and migration of PDL cells. Furthermore, the transplantation of MSC-protein enhanced periodontal tissue regeneration with the formation of new alveolar bone and PDLs. CONCLUSIONS:These results indicate that the MSC-protein promotes the proliferation and migration of PDL cells and induces significant periodontal tissue regeneration, suggesting that the MSC-protein could be used as a new cell-free treatment for periodontal disease. |
| Volume | 27(2) |
| Pages | 201-212 |
| Published | 2025-2-1 |
| DOI | 10.1016/j.jcyt.2024.10.003 |
| PII | S1465-3249(24)00892-2 |
| PMID | 39545910 |
| MeSH | Animals Cell Movement / drug effects Cell Proliferation / drug effects Fibroblast Growth Factor 2 / metabolism Fibroblast Growth Factor 2 / pharmacology Humans Male Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells* / cytology Mesenchymal Stem Cells* / metabolism Periodontal Diseases / therapy Periodontal Ligament* / cytology Periodontal Ligament* / drug effects Rats Regeneration* / drug effects |
| IF | 4.218 |
| Resource | |
| Human and Animal Cells | UE7T-13(RCB2161) |