Reference - Detail
| RRC ID | 82182 |
|---|---|
| Author | Pan ZQ, Yang YS, Du LL. |
| Title | Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe. |
| Journal | Methods Mol Biol |
| Abstract |
Protein-protein interactions play critical roles in biological processes. We previously developed the Pil1 co-tethering assay, an imaging-based method to detect protein-protein interactions in living Schizosaccharomyces pombe cells. This assay leverages the distinct localization pattern of the Pil1 protein by fusing a bait protein to Pil1 and examining whether a prey protein co-localize with the Pil1-fused bait. Here, we present an improved protocol of the Pil1 co-tethering assay. In this protocol, modified stable integration vectors (SIVs) with a NotI site as the linearization site are used to express bait and prey proteins. We expect that this protocol will enhance the application of the Pil1 co-tethering assay for studying protein-protein interactions. |
| Volume | 2862 |
| Pages | 93-102 |
| Published | 2025-1-1 |
| DOI | 10.1007/978-1-0716-4168-2_7 |
| PMID | 39527195 |
| MeSH | Genetic Vectors / genetics Genetic Vectors / metabolism Protein Binding Protein Interaction Mapping* / methods Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | |