Reference - Detail
| RRC ID | 82369 |
|---|---|
| Author | Yamada H, Kato N, Ichikawa M, Mannen K, Kiba T, Osakabe Y, Sakakibara H, Matsui M, Okamoto T. |
| Title | DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes from Recalcitrant Maize Inbred B73. |
| Journal | Plant Cell Physiol |
| Abstract |
Genome-editing tools such as the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system have become essential tools for increasing the efficiency and accuracy of plant breeding. Using such genome-editing tools on maize, one of the most important cereal crops of the world, will greatly benefit the agriculture and the mankind. Conventional genome-editing methods typically used for maize involve insertion of a Cas9-guide RNA expression cassette and a selectable marker in the genome DNA; however, using such methods, it is essential to eliminate the inserted DNA cassettes to avoid legislative concerns on gene-modified organisms. Another major hurdle for establishing an efficient and broadly applicable DNA-free genome-editing system for maize is presented by recalcitrant genotypes/cultivars, since cell/tissue culture and its subsequent regeneration into plantlets are crucial for producing transgenic and/or genome-edited maize. In this study, to establish a DNA-free genome-editing system for recalcitrant maize genotypes/cultivars, Cas9-gRNA ribonucleoproteins were directly delivered into zygotes isolated from the pollinated flowers of the maize-B73 cultivar. The zygotes successfully developed and were regenerated into genome-edited plantlets by co-culture with phytosulfokine, a peptide phytohormone. The method developed herein made it possible to obtain DNA- and selectable-marker-free genome-edited recalcitrant maize genotypes/cultivars with high efficiency. This method can advance the molecular breeding of maize and other important cereals, regardless of their recalcitrant characteristics. |
| Volume | 65(5) |
| Pages | 729-736 |
| Published | 2024-5-30 |
| DOI | 10.1093/pcp/pcae010 |
| PII | 7591713 |
| PMID | 38288629 |
| MeSH | CRISPR-Cas Systems* DNA, Plant / genetics Gene Editing* / methods Genome, Plant* Plant Breeding / methods Plants, Genetically Modified RNA, Guide, CRISPR-Cas Systems / genetics Zea mays* / genetics Zygote / metabolism |
| IF | 4.062 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00031 |