論文 - 詳細
| RRC ID | 82453 |
|---|---|
| 著者 | Ohishi H, Shinkai S, Owada H, Fujii T, Hosoda K, Onami S, Yamamoto T, Ohkawa Y, Ochiai H. |
| タイトル | Transcription-coupled changes in genomic region proximities during transcriptional bursting. |
| ジャーナル | Sci Adv |
| Abstract |
The orchestration of our genes heavily relies on coordinated communication between enhancers and promoters, yet the mechanisms behind this dynamic interplay during active transcription remain unclear. Here, we investigated enhancer-promoter (E-P) interactions in relation to transcriptional bursting in mouse embryonic stem cells using sequential DNA/RNA/immunofluorescence-fluorescence in situ hybridization analyses. Our data reveal that the active state of specific genes is characterized by specific proximities between different genomic regions and the accumulation of transcriptional regulatory factors. Mathematical simulations suggest that an increase in local viscosity could potentially contribute to stabilizing the duration of these E-P proximities. Our study provides insights into the association among E-P proximity, protein accumulation, and transcriptional dynamics, paving the way for a more nuanced understanding of gene-specific regulatory mechanisms. |
| 巻・号 | 10(49) |
| ページ | eadn0020 |
| 公開日 | 2024-12-6 |
| DOI | 10.1126/sciadv.adn0020 |
| PMID | 39642226 |
| PMC | PMC11623302 |
| MeSH | Animals Enhancer Elements, Genetic* Gene Expression Regulation Genome In Situ Hybridization, Fluorescence Mice Mouse Embryonic Stem Cells* / cytology Mouse Embryonic Stem Cells* / metabolism Promoter Regions, Genetic* Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic* |
| IF | 13.117 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 97 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | E14tg2a(AES0135) |