論文 - 詳細
| RRC ID | 82509 |
|---|---|
| 著者 | Ju M, Minami K, Katsuki S, Takenaka W, Tatekawa S, Tamari K, Koizumi M, Takahashi Y, Ogawa K. |
| タイトル | Synergistic Effects of Melatonin on Radiosensitization in Carbon-ion Radiotherapy. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Despite the established antitumor effectiveness and synergistic interactions of melatonin with photon irradiation, its role in carbon-ion radiotherapy remains uncertain. This study aimed to elucidate the mechanisms and potential clinical advantages of combining exogenous melatonin therapy with carbon-ion radiotherapy. MATERIALS AND METHODS:The investigation assessed the impact of combining exogenous melatonin with photon or carbon-ion irradiation on cell-cycle modulation and DNA-repair capability using the melanoma cell line B16F10. RNA sequencing and bioinformatics analysis were conducted to explore mechanisms and evaluate potential clinical benefits, with validation performed on the osteosarcoma cell line LM8. RESULTS:Pre-treatment with melatonin reduced the survival fraction of B16F10 and LM8 cells upon exposure to photon and carbon-ion radiation. Mechanistically, melatonin was found to inhibit G2/M arrest, preserve DNA damage, and suppress key genes involved in DNA double-strand break repair after 8 Gy carbon-ion radiation. Furthermore, RNA sequencing and bioinformatics analysis revealed favorable changes in genes associated with survival and metastasis, highlighting potential clinical significance. LM8 cells treated with melatonin exhibited increased radiosensitivity and suppression of DNA-repair proteins. CONCLUSION:The combination of exogenous melatonin not only heightened radiosensitivity and modulated hallmark tumor gene sets in vitro but also markedly suppressed the efficiency of DNA double-strand break-repair pathway, thus enhancing the cytotoxicity of carbon-ion radiotherapy. |
| 巻・号 | 44(8) |
| ページ | 3295-3306 |
| 公開日 | 2024-8-1 |
| DOI | 10.21873/anticanres.17148 |
| PII | 44/8/3295 |
| PMID | 39060064 |
| MeSH | Animals Cell Line, Tumor Cell Survival / drug effects Cell Survival / radiation effects DNA Breaks, Double-Stranded / drug effects DNA Breaks, Double-Stranded / radiation effects DNA Repair* / drug effects DNA Repair* / radiation effects Heavy Ion Radiotherapy* Humans Melanoma, Experimental / drug therapy Melanoma, Experimental / pathology Melanoma, Experimental / radiotherapy Melatonin* / pharmacology Mice Radiation Tolerance* / drug effects Radiation-Sensitizing Agents* / pharmacology |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | LM8(RCB1450) B16F10(RCB2630) |