RRC ID 82576
Author Stompor-Gorący M, Włoch A, Sengupta P, Nasulewicz-Goldeman A, Wietrzyk J.
Title Synergistic Proliferation Effects of Xanthohumol and Niflumic Acid on Merkel and Glioblastoma Cancer Cells: Role of Cell Membrane Interactions.
Journal Int J Mol Sci
Abstract The objective of our research was to determine the effects of xanthohumol (XN), a flavonoid isolated from hops (Humulus lupulus), and the anti-inflammatory drug niflumic acid (NA), separately and in combination with each other, on the proliferation of human cancer cells. Additionally, so as to understand the mechanism underlying the anticancer properties of the tested compounds, their effects on the biophysical parameters of a model membrane were assessed. The cells were incubated with XN and NA at various concentrations, either individually or in combination with each other. Cell proliferation was quantified using the sulforodamine B (SRB) assay. In addition, the IC50 values for niflumic acid and xanthohumol applied separately were determined by cell proliferation tests for the following human cancer cell lines: 5637 (urinary bladder carcinoma), A-431 (epidermoid carcinoma), UM-SCC-17A (head and neck squamous carcinoma), SK-MEL-3 (melanoma), MCC13 (Merkel cell cancer), and A172 (glioblastoma), in comparison with the mouse normal fibroblasts (BALB/3T3 clone A31). The results show that the two-compound combinations of XN and NA significantly decreased the proliferation of cancer cells in a dose-dependent manner, and the effects were stronger than the additive responses to XN and NA individually. The membrane studies revealed a synergistic effect on the membrane rigidity when using the mixture of XN and NA, which may explain the observed increase in anticancer activity for the combined XN and NA. Our results suggest that NSAIDs, such as niflumic acid, may be a promising strategy for co-application with xanthohumol as anticancer drugs.
Volume 25(20)
Published 2024-10-13
DOI 10.3390/ijms252011015
PII ijms252011015
PMID 39456799
PMC PMC11508127
MeSH Animals Antineoplastic Agents / pharmacology Cell Line, Tumor Cell Membrane* / drug effects Cell Membrane* / metabolism Cell Proliferation* / drug effects Drug Synergism* Flavonoids* / pharmacology Glioblastoma* / drug therapy Glioblastoma* / metabolism Glioblastoma* / pathology Humans Mice Niflumic Acid* / pharmacology Propiophenones* / pharmacology
IF 4.556
Resource
Human and Animal Cells 5637(RCB1191)