RRC ID 82637
Author Nishimoto S, Debarbat A, Ikeda Y, Arikawa E, Odagaki Y, Yano H, Qiao Y, Ito M, Kimura T, Takita T, Yasukawa K.
Title Expression of Recombinant Human α-Glucosidase in HEK293 Cells.
Journal J Agric Food Chem
Abstract In mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 °C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities but not sucrase activity, whereas the SI-expressing HEK293 cells extract (SIE) exhibited sucrase, isomaltase, and maltase activities but not glucoamylase activity. The apparent Km value of the MGE for maltose hydrolysis was 14-26% of that of the SIE for maltose, sucrose, and isomaltose hydrolysis. The respective apparent Vmax values of the MGE and SIE for sucrose and isomaltose hydrolysis were 0% and 6% and 10% and 42% of those for maltose hydrolysis. These results indicated that the maltase activities of MGAM and SI were higher than those of sucrase and isomaltase.
Published 2024-12-23
DOI 10.1021/acs.jafc.4c06902
PMID 39714016
Resource
DNA material pCMV_S-FLAG (RDB05956)