論文 - 詳細
| RRC ID | 82653 |
|---|---|
| 著者 | Katsuki S, Minami K, Oniwa K, Yagi M, Shimizu S, Hamatani N, Takashina M, Kanai T, Ogawa K. |
| タイトル | Ultra-high dose rate (FLASH) carbon ion irradiation inhibited immune suppressive protein expression on Pan02 cell line. |
| ジャーナル | J Radiat Res |
| Abstract |
Recently, ultra-high dose rate (> 40 Gy/s, uHDR; FLASH) radiation therapy (RT) has attracted interest, because the FLASH effect that is, while a cell-killing effect on cancer cells remains, the damage to normal tissue could be spared has been reported. This study aimed to compare the immune-related protein expression on cancer cells after γ-ray, conventionally used dose rate (Conv) carbon ion (C-ion), and uHDR C-ion. B16F10 murine melanoma and Pan02 murine pancreas cancer were irradiated with γ-ray at Osaka University and with C-ion at Osaka HIMAK. The dose rates at 1.16 Gy/s for Conv and 380 Gy/s for uHDR irradiation. The expressed calreticulin (CRT), major histocompatibility complex class (MHC)-I, and programmed cell death 1 ligand (PD-L1) were evaluated by flow cytometry. Western blotting and PCR were utilized to evaluate endoplasmic reticulum (ER) stress, DNA damage, and its repair pathway. CRT, MHC-I on B16F10 was also increased by irradiation, while only C-ion increased MHC-I on Pan02. Notably, PD-L1 on B16F10 was increased after irradiation with both γ-ray and C-ion, while uHDR C-ion suppressed the expression of PD-L1 on Pan02. The present study indicated that uHDR C-ion has a different impact on the repair pathway of DNA damage and ER than the Conv C-ion. This is the first study to show the immune-related protein expressions on cancer cells after uHDR C-ion irradiation. |
| 巻・号 | 66(1) |
| ページ | 97-102 |
| 公開日 | 2025-1-22 |
| DOI | 10.1093/jrr/rrae091 |
| PII | 7933256 |
| PMID | 39724928 |
| PMC | PMC11753840 |
| MeSH | Animals Calreticulin / metabolism Carbon* Cell Line, Tumor DNA Damage / radiation effects Dose-Response Relationship, Radiation Endoplasmic Reticulum Stress / radiation effects Gamma Rays Heavy Ion Radiotherapy* Mice |
| IF | 1.95 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16F10(RCB2630) |