| RRC ID |
82688
|
| 著者 |
Shohei Sakuda, Masaki Sunaoka, Maho Terada, Ayaka Sakoda, Natsumi Ishijima, Noriko Hakoshima, Kenichi Uchida, Hirofumi Enomoto, Tomohiro Furukawa
|
| タイトル |
Inhibition of Aflatoxin Production in Aspergillus flavus by a Klebsiella sp. and Its Metabolite Cyclo(l-Ala-Gly)
|
| ジャーナル |
Toxins
|
| Abstract |
During an experiment where we were cultivating aflatoxigenic Aspergillus flavus on peanuts, we accidentally discovered that a bacterium adhering to the peanut strongly inhibited aflatoxin (AF) production by A. flavus. The bacterium, isolated and identified as Klebsiella aerogenes, was found to produce an AF production inhibitor. Cyclo(l-Ala-Gly), isolated from the bacterial culture supernatant, was the main active component. The aflatoxin production-inhibitory activity of cyclo(l-Ala-Gly) has not been reported. Cyclo(l-Ala-Gly) inhibited AF production in A. flavus without affecting its fungal growth in a liquid medium with stronger potency than cyclo(l-Ala-l-Pro). Cyclo(l-Ala-Gly) has the strongest AF production-inhibitory activity among known AF production-inhibitory diketopiperazines. Related compounds in which the methyl moiety in cyclo(l-Ala-Gly) is replaced by ethyl, propyl, or isopropyl have shown much stronger activity than cyclo(l-Ala-Gly). Cyclo(l-Ala-Gly) did not inhibit recombinant glutathione-S-transferase (GST) in A. flavus, unlike (l-Ala-l-Pro), which showed that the inhibition of GST was not responsible for the AF production-inhibition of cyclo(l-Ala-Gly). When A. flavus was cultured on peanuts dipped for a short period of time in a dilution series bacterial culture broth, AF production in the peanuts was strongly inhibited, even at a 1 × 104-fold dilution. This strong inhibitory activity suggests that the bacterium is a candidate for an effective biocontrol agent for AF control.
|
| 巻・号 |
16
|
| ページ |
141
|
| 公開日 |
2024-3-8
|
| DOI |
10.3390/toxins16030141
|
| PMID |
38535807
|
| PMC |
PMC10973989
|
| MeSH |
Aflatoxins*
Arachis
Aspergillus flavus*
Dipeptides
Glutathione Transferase
Klebsiella
|
| IF |
3.531
|
| リソース情報 |
| 病原真核微生物 |
Aspergillus flavus IFM 47798 |
