| RRC ID |
82691
|
| Author |
Takeuchi K, Tabe S, Yamamoto Y, Takahashi K, Matsuo M, Ueno Y, Ohtsuka M, Morinaga S, Miyagi Y, Yamaguchi T, Tanimizu N, Taniguchi H.
|
| Title |
Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment.
|
| Journal |
STAR Protoc
|
| Abstract |
Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including heterogeneous cancer-associated fibroblasts (CAFs), using patient-derived PDAC cells and human-induced pluripotent cell-derived endothelial and mesenchymal cells. We also describe the procedure for analyzing FPCO characteristics. FPCO can provide a platform for establishing a reliable drug screening system. For complete details on the use and execution of this protocol, please refer to Takeuchi et al.1.
|
| Volume |
6(1)
|
| Pages |
103539
|
| Published |
2025-1-7
|
| DOI |
10.1016/j.xpro.2024.103539
|
| PII |
S2666-1667(24)00704-4
|
| PMID |
39772386
|
| Resource |
| Human and Animal Cells |
1383D6(HPS1006)
293T(RCB2202) |
| DNA material |
CSII-EF-MCS (RDB04378)
CSII-EF-MCS-IRES2-hKO1 (RDB07915)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
