| RRC ID |
82693
|
| Author |
Maegawa H, Kohashi M, Harada Y, Tanaka A, Kajiwara S, Fujimoto T, Atagi H, Kaneda K.
|
| Title |
Antitumor immunostimulatory effect via cell-killing action of a novel extracorporeal blood circulating photodynamic therapy system using 5-aminolevulinic acid.
|
| Journal |
Sci Rep
|
| Abstract |
This study investigated whether intravenous administration of tumor cells killed by photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) had antitumor effects on distal tumors. Furthermore, a novel extracorporeal blood circulating 5-ALA/PDT system was developed. 5-ALA/PDT- (low or high irradiation) or anticancer drug-treated cells were intravenously administered to rats in a glioma cancer model. CD8+ T cell infiltration into the tumor and expression of calreticulin were examined. The cell-killing effect in the circulating PDT system and protoporphyrin IX (PpIX) accumulation were evaluated. An antitumor effect was observed only with preadministration of low-irradiated 5-ALA/PDT-treated cells and was characterized by the infiltration of CD8+ T cells into the tumor. In low-irradiated cells, several types of cell death were observed, and cell surface calreticulin expression increased over time. A method for the intravenous administration of 5-ALA/PDT-treated cells along with extracorporeal blood circulation was then developed to target hematologic malignancies. Gradually cell death in the circulating PDT system and tumor-specific PpIX accumulation was confirmed using hematopoietic tumor cells. Thus, the extracorporeal blood circulating 5-ALA/PDT system has a direct cell-killing effect and an antitumor effect via induced immune activity and illustrates a new therapeutic strategy for hematologic malignancies.
|
| Volume |
15(1)
|
| Pages |
1064
|
| Published |
2025-1-7
|
| DOI |
10.1038/s41598-024-84861-8
|
| PII |
10.1038/s41598-024-84861-8
|
| PMID |
39775122
|
| PMC |
PMC11707032
|
| MeSH |
Aminolevulinic Acid* / pharmacology
Animals
CD8-Positive T-Lymphocytes / drug effects
CD8-Positive T-Lymphocytes / immunology
Calreticulin / metabolism
Cell Line, Tumor
Glioma* / drug therapy
Glioma* / pathology
Glioma* / therapy
Humans
Male
Photochemotherapy* / methods
Photosensitizing Agents* / pharmacology
Protoporphyrins* / pharmacology
Rats
|
| Resource |
| Human and Animal Cells |
ATN-1(RCB1440) |
