論文 - 詳細
| RRC ID | 82704 |
|---|---|
| 著者 | Toda E, Koshimizu S, Kinoshita A, Higashiyama T, Izawa T, Yano K, Okamoto T. |
| タイトル | Transcriptional dynamics during karyogamy in rice zygotes. |
| ジャーナル | Development |
| Abstract |
Upon fertilization, male and female nuclei fuse to form the zygotic nucleus in angiosperms. Karyogamy is considered to be essential for proper embryogenesis; however, the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant zygotes. The time-series RNA-seq analysis detected possible de novo and altered gene expression in zygotes from 15 min post-fertilization. Fertilization-induced transcription during karyogamy was characterized by protein interaction database and gene ontology analyses. Furthermore, paternal allele transcription was initiated approximately 30 min-1 h after gamete fusion, when nuclear fusion begins in the zygote. Some transcripts preferentially expressed in egg cells were downregulated after gamete fusion. Moreover, a dynamic shift from maternal-biased transcripts to bi-parental expression occurred during early zygotic development. These results suggest that transcriptional dynamics during karyogamy plays initial role in proper and sequential zygotic development and embryogenesis. |
| 公開日 | 2025-1-7 |
| DOI | 10.1242/dev.204497 |
| PII | 364937 |
| PMID | 39777484 |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00031 |