論文 - 詳細
| RRC ID | 82717 |
|---|---|
| 著者 | Kim N, Yokobayashi Y. |
| タイトル | Scalable control of stem cell fate by riboswitch-regulated RNA viral vector without genomic integration. |
| ジャーナル | Mol Ther |
| Abstract |
Transgene expression in stem cells is a powerful means of regulating cellular properties and differentiation into various cell types. However, existing vectors for transgene expression in stem cells suffer from limitations such as the need for genomic integration, the transient nature of gene expression, and the inability to temporally regulate transgene expression, which hinder biomedical and clinical applications. Here we report a new class of RNA virus-based vectors for scalable and integration-free transgene expression in mouse embryonic stem cells (mESCs). The vector is equipped with a small molecule-regulated riboswitch and a drug selection marker that allow temporal regulation of transgene expression and stable maintenance of the vector in proliferating stem cells. We demonstrated the utility of the vector by maintaining the pluripotency of mESCs in a differentiation induction medium by expressing Nanog and inducing myogenic differentiation by triggering Myod1 expression, without altering the mESC genome. |
| 公開日 | 2025-1-9 |
| DOI | 10.1016/j.ymthe.2025.01.005 |
| PII | S1525-0016(25)00009-7 |
| PMID | 39797398 |
| PMC | PMC11897756 |
| MeSH | Animals Cell Differentiation* / genetics Cell Line Gene Expression Regulation Genetic Vectors* / genetics Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Mice Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / metabolism MyoD Protein / genetics MyoD Protein / metabolism Nanog Homeobox Protein / genetics Nanog Homeobox Protein / metabolism RNA, Viral / genetics Riboswitch* / genetics Transgenes |
| IF | 8.986 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BRC6(AES0010) |
| 遺伝子材料 | pCAGGS-LIF (RDB09182) |