Reference - Detail
| RRC ID | 82723 |
|---|---|
| Author | Tokizawa M, Enomoto T, Ito H, Wu L, Kobayashi Y, Mora-Macías J, Armenta-Medina D, Iuchi S, Kobayashi M, Nomoto M, Tada Y, Fujita M, Shinozaki K, Yamamoto YY, Kochian LV, Koyama H. |
| Title | High affinity promoter binding of STOP1 is essential for early expression of novel aluminum-induced resistance genes GDH1 and GDH2 in Arabidopsis. |
| Journal | J Exp Bot |
| Abstract |
Malate efflux from roots, which is regulated by the transcription factor STOP1 (SENSITIVE-TO-PROTON-RHIZOTOXICITY1) and mediates aluminum-induced expression of ALUMINUM-ACTIVATED-MALATE-TRANSPORTER1 (AtALMT1), is critical for aluminum resistance in Arabidopsis thaliana. Several studies showed that AtALMT1 expression in roots is rapidly observed in response to aluminum; this early induction is an important mechanism to immediately protect roots from aluminum toxicity. Identifying the molecular mechanisms that underlie rapid aluminum resistance responses should lead to a better understanding of plant aluminum sensing and signal transduction mechanisms. In this study, we observed that GFP-tagged STOP1 proteins accumulated in the nucleus soon after aluminum treatment. The rapid aluminum-induced STOP1-nuclear localization and AtALMT1 induction were detected in the presence of a protein synthesis inhibitor, suggesting that post-translational regulation is involved in these events. STOP1 also regulated rapid aluminum-induced expression for other genes that carry a functional/high-affinity STOP1-binding site in their promoter, including STOP2, GLUTAMATE-DEHYDROGENASE1 and 2 (GDH1 and 2). However STOP1 did not regulate Al resistance genes which have no functional STOP1-binding site such as ALUMINUM-SENSITIVE3, suggesting that the binding of STOP1 in the promoter is essential for early induction. Finally, we report that GDH1 and 2 which are targets of STOP1, are novel aluminum-resistance genes in Arabidopsis. |
| Volume | 72(7) |
| Pages | 2769-2789 |
| Published | 2021-3-29 |
| DOI | 10.1093/jxb/erab031 |
| PII | 6106577 |
| PMID | 33481007 |
| MeSH | Aluminum / metabolism Aluminum / toxicity* Arabidopsis* / genetics Arabidopsis* / metabolism Arabidopsis Proteins* / genetics Arabidopsis Proteins* / metabolism Gene Expression Regulation, Plant Glutamate Dehydrogenase Plant Roots / genetics Plant Roots / metabolism Promoter Regions, Genetic Transcription Factors / metabolism |
| IF | 5.908 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | sja05800 |