論文 - 詳細
| RRC ID | 82732 |
|---|---|
| 著者 | He L, Lin J, Qin Z, Xu Q, Hao L, Fu Y, Ran X, Chen W. |
| タイトル | Long non-coding RNA NEAT1 promotes ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis. |
| ジャーナル | J Ovarian Res |
| Abstract |
BACKGROUND:Granulosa cell proliferation and survival are essential for normal ovarian function and follicular development. Long non-coding RNAs (lncRNAs) have emerged as important regulators of cell proliferation and differentiation. Nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated in various cellular processes, but its role in granulosa cell function remains unclear. METHODS:We investigated the function of lncRNA NEAT1 in human ovarian granulosa-like tumor cells (KGN). The effects of NEAT1 overexpression or silencing on cell proliferation and cell cycle were evaluated using CCK-8 assays and flow cytometry. The interaction between NEAT1, miR-29a-3p, and IGF1 was examined using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis. RESULTS:NEAT1 promoted granulosa cell proliferation and cell cycle progression by indirectly upregulated IGF1 expression through acting as a molecular sponge for miR-29a-3p. Cell proliferation and G2/M phase proportions were increased by overexpression of NEAT1, whereas cell proliferation and G2/M phase proportions decreased with NEAT1 silencing. The effects of NEAT1 on cell proliferation and cell cycle-related proteins (CCNB1 and CDK2) were partially reversed by miR-29a-3p mimic, while miR-29a-3p inhibitor rescued the effects of NEAT1 silencing. CONCLUSION:LncRNA NEAT1 could promote ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis in polycystic ovary syndrome. Further investigation of this mechanism in clinical samples may have implications for understanding ovarian physiology and pathology. |
| 巻・号 | 18(1) |
| ページ | 6 |
| 公開日 | 2025-1-13 |
| DOI | 10.1186/s13048-025-01588-4 |
| PII | 10.1186/s13048-025-01588-4 |
| PMID | 39806494 |
| PMC | PMC11727426 |
| MeSH | Cell Cycle* / genetics Cell Line, Tumor Cell Proliferation* / genetics Female Granulosa Cells* / metabolism Humans Insulin-Like Growth Factor I* / genetics Insulin-Like Growth Factor I* / metabolism MicroRNAs* / genetics MicroRNAs* / metabolism RNA, Long Noncoding* / genetics RNA, Long Noncoding* / metabolism |
| IF | 2.469 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |