RRC ID 82732
Author He L, Lin J, Qin Z, Xu Q, Hao L, Fu Y, Ran X, Chen W.
Title Long non-coding RNA NEAT1 promotes ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis.
Journal J Ovarian Res
Abstract BACKGROUND:Granulosa cell proliferation and survival are essential for normal ovarian function and follicular development. Long non-coding RNAs (lncRNAs) have emerged as important regulators of cell proliferation and differentiation. Nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated in various cellular processes, but its role in granulosa cell function remains unclear.
METHODS:We investigated the function of lncRNA NEAT1 in human ovarian granulosa-like tumor cells (KGN). The effects of NEAT1 overexpression or silencing on cell proliferation and cell cycle were evaluated using CCK-8 assays and flow cytometry. The interaction between NEAT1, miR-29a-3p, and IGF1 was examined using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis.
RESULTS:NEAT1 promoted granulosa cell proliferation and cell cycle progression by indirectly upregulated IGF1 expression through acting as a molecular sponge for miR-29a-3p. Cell proliferation and G2/M phase proportions were increased by overexpression of NEAT1, whereas cell proliferation and G2/M phase proportions decreased with NEAT1 silencing. The effects of NEAT1 on cell proliferation and cell cycle-related proteins (CCNB1 and CDK2) were partially reversed by miR-29a-3p mimic, while miR-29a-3p inhibitor rescued the effects of NEAT1 silencing.
CONCLUSION:LncRNA NEAT1 could promote ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis in polycystic ovary syndrome. Further investigation of this mechanism in clinical samples may have implications for understanding ovarian physiology and pathology.
Volume 18(1)
Pages 6
Published 2025-1-13
DOI 10.1186/s13048-025-01588-4
PII 10.1186/s13048-025-01588-4
PMID 39806494
PMC PMC11727426
MeSH Cell Cycle* / genetics Cell Line, Tumor Cell Proliferation* / genetics Female Granulosa Cells* / metabolism Humans Insulin-Like Growth Factor I* / genetics Insulin-Like Growth Factor I* / metabolism MicroRNAs* / genetics MicroRNAs* / metabolism RNA, Long Noncoding* / genetics RNA, Long Noncoding* / metabolism
Resource
Human and Animal Cells KGN(RCB1154)