| RRC ID |
82785
|
| Author |
Sauteur L, Krudewig A, Herwig L, Ehrenfeuchter N, Lenard A, Affolter M, Belting HG.
|
| Title |
Cdh5/VE-cadherin promotes endothelial cell interface elongation via cortical actin polymerization during angiogenic sprouting.
|
| Journal |
Cell Rep
|
| Abstract |
Organ morphogenesis requires the coordination of cell behaviors. Here, we have analyzed dynamic endothelial cell behaviors underlying sprouting angiogenesis in vivo. Two different mechanisms contribute to sprout outgrowth: tip cells show strong migratory behavior, whereas extension of the stalk is dependent upon cell elongation. To investigate the function of Cdh5 in sprout outgrowth, we generated null mutations in the zebrafish cdh5 gene, and we found that junctional remodeling and cell elongation are impaired in mutant embryos. The defects are associated with a disorganization of the actin cytoskeleton and cannot be rescued by expression of a truncated version of Cdh5. Finally, the defects in junctional remodeling can be phenocopied by pharmacological inhibition of actin polymerization, but not by inhibiting actin-myosin contractility. Taken together, our results support a model in which Cdh5 organizes junctional and cortical actin cytoskeletons, as well as provides structural support for polymerizing F-actin cables during endothelial cell elongation.
|
| Volume |
9(2)
|
| Pages |
504-13
|
| Published |
2014-10-23
|
| DOI |
10.1016/j.celrep.2014.09.024
|
| PII |
S2211-1247(14)00803-1
|
| PMID |
25373898
|
| MeSH |
Actin Cytoskeleton / metabolism
Actins / metabolism*
Animals
Antigens, CD / genetics
Antigens, CD / metabolism*
Cadherins / genetics
Cadherins / metabolism*
Cell Movement
Endothelial Cells / cytology
Endothelial Cells / metabolism*
Endothelial Cells / physiology
Endothelium, Vascular / embryology
Endothelium, Vascular / metabolism
Intercellular Junctions / metabolism
Myosins / metabolism
Neovascularization, Physiologic*
Polymerization
Zebrafish / embryology
Zebrafish / genetics
Zebrafish / metabolism*
|
| IF |
8.109
|
| Resource |
| Zebrafish |
UAS:RFP |
