RRC ID 82800
Author Dong Z, Peng J, Guo S.
Title Stable gene silencing in zebrafish with spatiotemporally targetable RNA interference.
Journal Genetics
Abstract The ability to regulate gene activity in a spatiotemporally controllable manner is vital for biological discovery that will impact disease diagnosis and treatment. While conditional gene silencing is possible in other genetic model organisms, this technology is largely unavailable in zebrafish, an important vertebrate model organism for functional gene discovery. Here, using short hairpin RNAs (shRNAs) designed in the microRNA-30 backbone, which have been shown to mimic natural microRNA primary transcripts and be more effective than simple shRNAs, we report stable RNA interference-mediated gene silencing in zebrafish employing the yeast Gal4-UAS system. Using this approach, we reveal at single-cell resolution the role of atypical protein kinase Cλ (aPKCλ) in regulating neural progenitor/stem cell division. We also show effective silencing of the one-eyed-pinhead and no-tail/brachyury genes. Furthermore, we demonstrate stable integration and germ-line transmission of the UAS-miR-shRNAs for aPKCλ, the expressivity of which is controllable by the strength and expression of Gal4. This technology shall significantly advance the utility of zebrafish for understanding fundamental vertebrate biology and for the identification and evaluation of important therapeutic targets.
Volume 193(4)
Pages 1065-71
Published 2013-4-1
DOI 10.1534/genetics.112.147892
PII genetics.112.147892
PMID 23378068
PMC PMC3606086
MeSH Activating Transcription Factors / metabolism Animals Brachyury Protein Cell Division Enhancer Elements, Genetic Fetal Proteins / genetics Fetal Proteins / metabolism Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism Isoenzymes / genetics Isoenzymes / metabolism MicroRNAs / genetics MicroRNAs / metabolism Neural Stem Cells / metabolism Protein Kinase C / genetics Protein Kinase C / metabolism RNA Interference* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism T-Box Domain Proteins / genetics T-Box Domain Proteins / metabolism Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism
IF 4.015
Resource
Zebrafish SAGFF(LF)73A