| RRC ID |
82816
|
| Author |
Hattori M, Wazawa T, Orioka M, Hiruta Y, Nagai T.
|
| Title |
Creating coveted bioluminescence colors for simultaneous multi-color bioimaging.
|
| Journal |
Sci Adv
|
| Abstract |
Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena.
|
| Volume |
11(4)
|
| Pages |
eadp4750
|
| Published |
2025-1-24
|
| DOI |
10.1126/sciadv.adp4750
|
| PMID |
39841832
|
| PMC |
PMC11753369
|
| MeSH |
Animals
Color*
Humans
Luminescent Measurements* / methods
Luminescent Proteins / chemistry
Luminescent Proteins / metabolism
|
| IF |
13.117
|
| Resource |
| DNA material |
pcDNA3 Venus-Akaluc (RDB15781) |
| Human and Animal Cells |
C2C12(RCB0987)
293(RCB1637)
HeLa |
