| RRC ID |
82834
|
| Author |
Ogura K, Kawashima I, Kasahara K.
|
| Title |
HGS Promotes Tumor Growth, Whereas the Coiled-Coil Domain and Its Oligopeptide of HGS Suppress It.
|
| Journal |
Int J Mol Sci
|
| Abstract |
We previously isolated a cDNA clone for galactosylceramide expression factor 1, which is the rat homologue of hepatocyte-growth-factor-regulated tyrosine kinase substrate (HGS) and induces galactosylceramide expression and morphological changes in COS-7 cells, and reported that overexpression of HGS induced morphological changes in canine kidney epithelial MDCK cells. HGS is a component of the endosomal sorting complexes required for transport machinery that mediates endosomal multivesicle body formation. In this study, the overexpression of HGS induced epithelial-mesenchymal transition and caused transformation in MDCK cells, whereas the overexpression of a coiled-coil domain of HGS inhibited induction of epithelial-mesenchymal transition by HGF stimulation. The overexpression of HGS in mouse melanoma B16 cells and human colorectal cancer COLO205 cells promoted cancer characteristic anchorage-independent cell growth ability and tumor growth, whereas the overexpression of the coiled-coil domain of HGS in these cells suppressed them. The oligopeptide OP12-462 constituting the coiled-coil domain suppressed the anchorage-independent cell growth ability and tumor growth of COLO205 cells. The coiled-coil domain of HGS and OP12-462 are novel tumor growth inhibitors that do not directly destroy cancer cells but rather inhibit only the anchorage-independent cell growth ability of cancer cells.
|
| Volume |
26(2)
|
| Published |
2025-1-17
|
| DOI |
10.3390/ijms26020772
|
| PII |
ijms26020772
|
| PMID |
39859488
|
| PMC |
PMC11766344
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation*
Dogs
Epithelial-Mesenchymal Transition / genetics
Humans
Madin Darby Canine Kidney Cells
Mice
Oligopeptides* / metabolism
Oligopeptides* / pharmacology
Protein Domains
Rats
|
| IF |
4.556
|
| Resource |
| Human and Animal Cells |
MDCK(RCB0995)
COLO205(RCB2127) |
| DNA material |
pSmad RE-TK hRluc(F) (RDB05622) |
