Reference - Detail
| RRC ID | 82853 |
|---|---|
| Author | Jung J, Park J, Park S, Kim CH, Jung H. |
| Title | Protocadherin 19 regulates axon guidance in the developing Xenopus retinotectal pathway. |
| Journal | Mol Brain |
| Abstract |
Protocadherin 19 (Pcdh19) is a homophilic cell adhesion molecule and is involved in a variety of neuronal functions. Here, we tested whether Pcdh19 has a regulatory role in axon guidance using the developing Xenopus retinotectal system. We performed targeted microinjections of a translation blocking antisense morpholino oligonucleotide to knock down the expression of Pcdh19 selectively in the central nervous system. Knocking down Pcdh19 expression resulted in navigational errors of retinal ganglion cell (RGC) axons specifically at the optic chiasm. Instead of projecting to the contralateral optic tectum, RGC axons in the Pcdh19-depleted embryo misprojected ipsilaterally. Although incorrectly delivered into the ipsilateral brain hemisphere, these axons correctly reached the optic tectum. These data suggest that Pcdh19 has a critical role in preventing mixing of RGC axons originating from the opposite eyes at the optic chiasm, highlighting the importance of cell adhesion in bundling of RGC axons. |
| Volume | 17(1) |
| Pages | 58 |
| Published | 2024-8-22 |
| DOI | 10.1186/s13041-024-01130-5 |
| PII | 10.1186/s13041-024-01130-5 |
| PMID | 39175067 |
| PMC | PMC11342623 |
| MeSH | Animals Axon Guidance* Axons* / metabolism Cadherins* / metabolism Gene Expression Regulation, Developmental Gene Knockdown Techniques Optic Chiasm / embryology Optic Chiasm / metabolism Protocadherins* Retina / embryology Retina / metabolism Retinal Ganglion Cells* / metabolism Superior Colliculi / embryology Superior Colliculi / metabolism Visual Pathways Xenopus Proteins* / genetics Xenopus Proteins* / metabolism Xenopus laevis* / embryology |
| IF | 4.686 |
| Resource | |
| Clawed frogs / Newts | Xenopus tropicalis |