論文 - 詳細
| RRC ID | 83092 |
|---|---|
| 著者 | Kawakami K, Largaespada DA, Ivics Z. |
| タイトル | Transposons As Tools for Functional Genomics in Vertebrate Models. |
| ジャーナル | Trends Genet |
| Abstract |
Genetic tools and mutagenesis strategies based on transposable elements are currently under development with a vision to link primary DNA sequence information to gene functions in vertebrate models. By virtue of their inherent capacity to insert into DNA, transposons can be developed into powerful tools for chromosomal manipulations. Transposon-based forward mutagenesis screens have numerous advantages including high throughput, easy identification of mutated alleles, and providing insight into genetic networks and pathways based on phenotypes. For example, the Sleeping Beauty transposon has become highly instrumental to induce tumors in experimental animals in a tissue-specific manner with the aim of uncovering the genetic basis of diverse cancers. Here, we describe a battery of mutagenic cassettes that can be applied in conjunction with transposon vectors to mutagenize genes, and highlight versatile experimental strategies for the generation of engineered chromosomes for loss-of-function as well as gain-of-function mutagenesis for functional gene annotation in vertebrate models, including zebrafish, mice, and rats. |
| 巻・号 | 33(11) |
| ページ | 784-801 |
| 公開日 | 2017-11-1 |
| DOI | 10.1016/j.tig.2017.07.006 |
| PII | S0168-9525(17)30123-3 |
| PMID | 28888423 |
| PMC | PMC5682939 |
| MeSH | Animals DNA Transposable Elements* Genomics* Models, Genetic* Vertebrates / genetics* |
| IF | 11.333 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ゼブラフィッシュ | gSAIzGFFD478A, SAGFF(LF)128A, SAGFF(LF)234A, gSAGFF202A, HGn21A, gSAIzGFFD164A, SAIGFF213A, USA:GFP |