論文 - 詳細
| RRC ID | 83093 |
|---|---|
| 著者 | Lai S, Kumari A, Liu J, Zhang Y, Zhang W, Yen K, Xu J. |
| タイトル | Chemical screening reveals Ronidazole is a superior prodrug to Metronidazole for nitroreductase-induced cell ablation system in zebrafish larvae. |
| ジャーナル | J Genet Genomics |
| Abstract |
The Metronidazole (MTZ)/nitroreductase (NTR)-mediated cell ablation system is the most commonly used chemical-genetic cell ablation method in zebrafish. This system can specifically ablate target cells under spatial and temporal control. The MTZ/NTR system has become a widely used cell ablation system in biological, developmental, and functional studies. However, the inadequate cell-ablation ability of some cell types and the side effects of high concentration MTZ impede extensive applications of the MTZ/NTR system. In the present study, the US drug collection library was searched to extend the NTR system. Six MTZ analogs were found, and the cell-ablation ability of these analogs was tested in zebrafish larvae. The results revealed that two of the NTR substrates, Furazolidone and Ronidazole, ablated target cells more efficiently than MTZ at lower concentrations. Furthermore, the working concentration of Ronidazole, but not Furazolidone and MTZ, did not affect axonal bridge formation during spinal cord regeneration. Our results, taken together, indicate that Ronidazole is a superior prodrug to MTZ for the NTR system, especially for the study of neuron regeneration in zebrafish larvae. |
| 巻・号 | 48(12) |
| ページ | 1081-1090 |
| 公開日 | 2021-12-1 |
| DOI | 10.1016/j.jgg.2021.07.015 |
| PII | S1673-8527(21)00246-0 |
| PMID | 34411714 |
| MeSH | Animals Animals, Genetically Modified Larva / metabolism Metronidazole / metabolism Metronidazole / pharmacology Nitroreductases / genetics Nitroreductases / metabolism Prodrugs* / metabolism Prodrugs* / pharmacology Ronidazole Zebrafish* / metabolism |
| IF | 5.065 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | hspGFF62A |