論文 - 詳細
| RRC ID | 83183 |
|---|---|
| 著者 | Nagao R, Ogawa H, Suzuki T, Dohmae N, Kato K, Nakajima Y, Shen JR. |
| タイトル | Biochemical evidence for the diversity of LHCI proteins in PSI-LHCI from the red alga Galdieria sulphuraria NIES-3638. |
| ジャーナル | Photosynth Res |
| Abstract |
Red algae are photosynthetic eukaryotes whose light-harvesting complexes (LHCs) associate with photosystem I (PSI). In this study, we examined characteristics of PSI-LHCI, PSI, and LHCI isolated from the red alga Galdieria sulphuraria NIES-3638. The PSI-LHCI supercomplexes were purified using anion-exchange chromatography followed by hydrophobic-interaction chromatography, and finally by trehalose density gradient centrifugation. PSI and LHCI were similarly prepared following the dissociation of PSI-LHCI with Anzergent 3-16. Polypeptide analysis of PSI-LHCI revealed the presence of PSI and LHC proteins, along with red-lineage chlorophyll a/b-binding-like protein (RedCAP), which is distinct from LHC proteins within the LHC protein superfamily. RedCAP, rather than LHC proteins, exhibited tight binding to PSI. Carotenoid analysis of LHCI identified zeaxanthin, β-cryptoxanthin, and β-carotene, with zeaxanthin particularly enriched, which is consistent with other red algal LHCIs. A Qy peak of chlorophyll a in the LHCI absorption spectrum was blue-shifted compared with those of PSI-LHCI and PSI, and a fluorescence emission peak was similarly shifted to shorter wavelengths. Based on these results, we discuss the diversity of LHC proteins and RedCAP in red algal PSI-LHCI supercomplexes. |
| 巻・号 | 163(1) |
| ページ | 14 |
| 公開日 | 2025-1-27 |
| DOI | 10.1007/s11120-024-01134-1 |
| PII | 10.1007/s11120-024-01134-1 |
| PMID | 39870974 |
| MeSH | Carotenoids / metabolism Chlorophyll / metabolism Light-Harvesting Protein Complexes* / chemistry Light-Harvesting Protein Complexes* / isolation & purification Light-Harvesting Protein Complexes* / metabolism Photosystem I Protein Complex* / chemistry Photosystem I Protein Complex* / metabolism Rhodophyta* / metabolism |
| IF | 3.216 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-3638 |