論文 - 詳細
| RRC ID | 83219 |
|---|---|
| 著者 | Tanaka M, Fujii S, Inoue H, Takahashi N, Ishimi Y, Uehara M. |
| タイトル | (S)-Equol Is More Effective than (R)-Equol in Inhibiting Osteoclast Formation and Enhancing Osteoclast Apoptosis, and Reduces Estrogen Deficiency-Induced Bone Loss in Mice. |
| ジャーナル | J Nutr |
| Abstract |
BACKGROUND:Equol, a metabolite of daidzein, binds to the estrogen receptor with greater affinity than daidzein and exhibits various biological properties. It exists as an enantiomer, either (S)-equol or (R)-equol. OBJECTIVES:We have previously shown that the inhibitory effect of (S)-equol on bone fragility is stronger than that of racemic equol in ovariectomized (OVX) mice; however, the effect of (R)-equol has not been elucidated. The aim of this study was to compare the activities of equol enantiomers on bone metabolism in vitro and in vivo. METHODS:Bone marrow cells (BMCs) and RAW 264.7 cells were treated with equol enantiomers. The number of osteoclasts and caspase-3/7 activity were measured. We examined the effect of equol enantiomers on osteoblast differentiation in MC3T3-E1 cells. In vivo, 8-wk-old female ddY mice were assigned to 4 groups: sham-operated (sham), OVX, OVX + 0.5 mg/d of (S)-equol (S-eq), and OVX + 0.5 mg/d of (R)-equol (R-eq). Four weeks after the intervention, femoral bone mineral density (BMD) and osteoclastic gene expression were analyzed, along with concentrations of equol enantiomers in the serum and tissues. RESULTS:(S)-equol and (R)-equol inhibited osteoclast differentiation in BMCs (97% and 60%, P < 0.05) and RAW 264.7 cells (83% and 68%, P < 0.05). (S)-equol promoted apoptosis of mature osteoclasts by inducing caspase-3/7 activity (29%, P < 0.05) and enhanced osteoblast differentiation (29%, P < 0.05). In OVX mice, BMD was ameliorated in (S)-equol-treated mice (11%, P < 0.05), but not in (R)-equol-treated mice. The concentrations of (S)-equol were greater than those of (R)-equol in the serum, tibia, liver, and kidney (by 148%, 80%, 22%, and 139%, respectively). CONCLUSIONS:These results suggest that (S)-equol is more effective than (R)-equol in inhibiting osteoclast formation and enhancing osteoclast apoptosis in vitro, supporting the beneficial effect of (S)-equol to reduce estrogen deficiency-induced bone loss in OVX mice. |
| 巻・号 | 152(8) |
| ページ | 1831-1842 |
| 公開日 | 2022-8-9 |
| DOI | 10.1093/jn/nxac130 |
| PII | S0022-3166(22)00701-5 |
| PMID | 35675296 |
| MeSH | Animals Apoptosis Bone Density Bone Diseases, Metabolic* Bone Resorption* / drug therapy Bone Resorption* / prevention & control Caspase 3 Caspase 7 Equol / pharmacology Equol / therapeutic use Estrogens / pharmacology Female Mice Mice, Inbred Strains Osteoclasts Ovariectomy |
| IF | 4.281 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |