| RRC ID |
83228
|
| Author |
Kaneda Y, Lu Y, Sun J, Shimada K, Emori C, Noda T, Koyano T, Matsuyama M, Miyata H, Ikawa M.
|
| Title |
TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Sperm morphogenesis is a tightly regulated differentiation process, disruption of which leads to sperm malfunction and male infertility. Here, we show that Tex38 knockout (KO) male mice are infertile. Tex38 KO spermatids exhibit excess retention of residual cytoplasm around the head, resulting in abnormal sperm morphology with backward head bending. TEX38 interacts and colocalizes with ZDHHC19, a testis-enriched acyltransferase catalyzing protein S-palmitoylation, at the plasma membrane of spermatids. ZDHHC19 and TEX38 are each downregulated in mouse testes lacking the other protein. TEX38 stabilizes and localizes ZDHHC19 to the plasma membrane of cultured cells and vice versa, consolidating their interdependence. Mice deficient in ZDHHC19 or harboring a C142S mutation that disables the palmitoyltransferase activity of ZDHHC19 display phenotypes resembling those of Tex38 KO mice. Strikingly, ZDHHC19 palmitoylates ARRDC5, an arrestin family protein regulating sperm differentiation. Overall, our findings indicate that TEX38 forms a stable complex with ZDHHC19 at the plasma membrane of spermatids, which governs downstream S-palmitoylation of proteins essential for morphological transformation of spermatids.
|
| Volume |
122(10)
|
| Pages |
e2417943122
|
| Published |
2025-3-11
|
| DOI |
10.1073/pnas.2417943122
|
| PMID |
40030029
|
| MeSH |
Acyltransferases* / genetics
Acyltransferases* / metabolism
Animals
Cell Membrane* / metabolism
Infertility, Male / genetics
Infertility, Male / metabolism
Lipoylation*
Male
Mice
Mice, Knockout*
Morphogenesis
Sperm Head* / metabolism
Spermatids* / metabolism
Spermatogenesis / genetics
Spermatogenesis / physiology
Testis / metabolism
|
| IF |
9.412
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
