| RRC ID |
83292
|
| 著者 |
Shima Y, Skibbe H, Sasagawa Y, Fujimori N, Iwayama Y, Isomura-Matoba A, Yano M, Ichikawa T, Nikaido I, Hattori N, Kato T.
|
| タイトル |
Distinctiveness and continuity in transcriptome and connectivity in the anterior-posterior axis of the paraventricular nucleus of the thalamus.
|
| ジャーナル |
Cell Rep
|
| Abstract |
The paraventricular nucleus of the thalamus (PVT) projects axons to multiple areas, mediates a wide range of behaviors, and exhibits regional heterogeneity in both functions and axonal projections. Still, questions regarding the cell types present in the PVT and the extent of their differences remain inadequately addressed. We applied single-cell RNA sequencing to depict the transcriptomic characteristics of mouse PVT neurons. We found that one of the most significant variances in the PVT transcriptome corresponded to the anterior-posterior axis. While the single-cell transcriptome classified PVT neurons into five types, our transcriptomic and histological analyses showed continuity among the cell types. We discovered that anterior and posterior subpopulations had nearly non-overlapping projection patterns, while another population showed intermediate patterns. In addition, these subpopulations responded differently to appetite-related neuropeptides, with their activation showing opposing effects on food consumption. Our studies unveiled the contrasts and the continuity of PVT neurons that underpin their function.
|
| 巻・号 |
42(10)
|
| ページ |
113309
|
| 公開日 |
2023-10-31
|
| DOI |
10.1016/j.celrep.2023.113309
|
| PII |
S2211-1247(23)01321-9
|
| PMID |
37862168
|
| MeSH |
Animals
Mice
Midline Thalamic Nuclei* / physiology
Paraventricular Hypothalamic Nucleus* / physiology
Thalamus
Transcriptome / genetics
|
| IF |
8.109
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
