Reference - Detail
| RRC ID | 83316 |
|---|---|
| Author | Ishikawa H, Kuno Y, Yokoo T, Nagashima R, Takaki T, Sasaki H, Kohda C, Iyoda M. |
| Title | In vitro investigation of the effects of Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 exopolysaccharides on tight junction damage caused by influenza virus infection. |
| Journal | Lett Appl Microbiol |
| Abstract |
It is a problem that influenza virus infection increases susceptibility to secondary bacterial infection in lungs leading to lethal pneumonia. We previously reported that exopolysaccharides (EPS) derived from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (OLL1073R-1) could prevent against influenza virus infection followed by secondary bacterial infection in vitro. Therefore, the present study assessed whether EPS derived OLL1073R-1 protects the alveolar epithelial barrier disfunction caused by influenza virus infection. After A549 cells treated with EPS or without EPS were infected influenza virus A/Puerto Rico/8/34 (IFV) for 12 h, the levels of tight junction genes expression and inflammatory genes expression were measured by reverse transcription polymerase chain reaction. As results, EPS treatment could protect against low-titer IFV infection, but not high-titer IFV infection, followed by suppression of the increased expression of inflammatory cytokine gene levels and recovery of the decrease in the expression level of ZO-1 gene that was caused by low-titer IFV infection, leading to an improvement trend in the barrier function. Our findings showed that EPS derived from OLL1073R-1 could inhibit low-titer IFV infection leading to maintenance of the epithelial barrier function through the suppression of inflammatory cytokine genes expression. |
| Volume | 77(4) |
| Published | 2024-4-8 |
| DOI | 10.1093/lambio/ovae029 |
| PII | 7634354 |
| PMID | 38521981 |
| MeSH | Bacterial Infections* Cytokines / genetics Cytokines / metabolism Humans Influenza, Human* Lactobacillus delbrueckii* / genetics Lactobacillus delbrueckii* / metabolism Orthomyxoviridae* Tight Junctions |
| IF | 2.173 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | A549 |