Reference - Detail
| RRC ID | 83492 |
|---|---|
| Author | Guo Y, Kitano T, Inoue K, Murano K, Hirose M, Li TD, Sakashita A, Ishizu H, Ogonuki N, Matoba S, Sato M, Ogura A, Siomi H. |
| Title | Obox4 promotes zygotic genome activation upon loss of Dux. |
| Journal | Elife |
| Abstract |
Once fertilized, mouse zygotes rapidly proceed to zygotic genome activation (ZGA), during which long terminal repeats (LTRs) of murine endogenous retroviruses with leucine tRNA primer (MERVL) are activated by a conserved homeodomain-containing transcription factor, DUX. However, Dux-knockout embryos produce fertile mice, suggesting that ZGA is redundantly driven by an unknown factor(s). Here, we present multiple lines of evidence that the multicopy homeobox gene, Obox4, encodes a transcription factor that is highly expressed in mouse two-cell embryos and redundantly drives ZGA. Genome-wide profiling revealed that OBOX4 specifically binds and activates MERVL LTRs as well as a subset of murine endogenous retroviruses with lysine tRNA primer (MERVK) LTRs. Depletion of Obox4 is tolerated by embryogenesis, whereas concomitant Obox4/Dux depletion markedly compromises embryonic development. Our study identified OBOX4 as a transcription factor that provides genetic redundancy to preimplantation development. |
| Volume | 13 |
| Published | 2024-6-24 |
| DOI | 10.7554/eLife.95856 |
| PII | 95856 |
| PMID | 38856708 |
| PMC | PMC11196112 |
| MeSH | Animals Embryonic Development / genetics Gene Expression Regulation, Developmental Genome Homeodomain Proteins* / genetics Homeodomain Proteins* / metabolism Mice Mice, Knockout Zygote* / metabolism |
| IF | 7.08 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | EB3(AES0139) |