論文 - 詳細
| RRC ID | 83497 |
|---|---|
| 著者 | Misaizu R, Endo-Takahashi Y, Nirasawa K, Negishi Y, Asayama S. |
| タイトル | Diffusive delivery of plasmid DNA using zwitterionic carboxyalkyl poly(1-vinylimidazole) into skeletal muscle in vivo. |
| ジャーナル | Biomater Sci |
| Abstract |
Zwitterionic carboxyalkyl poly(1-vinylimidazole) (CA-PVIm) polymers with imidazolium cations and carboxylate anions have been synthesized as a carrier for the in vivo delivery of plasmid DNA (pDNA) to skeletal muscle. From differential scanning calorimetry measurements, resulting CA-PVIm had intermediate water in hydration water as a biocompatible polymer. Notably, when the pDNA and resulting CA-PVIm were mixed, slight retarded bands of the pDNA were observed in agarose gel electrophoresis, suggesting the polyion complex (PIC) formation between the pDNA and CA-PVIm despite zwitterionic polymers. Resulting PICs maintained the higher-order structure of the pDNA. Using resulting pDNA PICs, the highest pDNA expression by intramuscular injection was achieved in the PIC with 7 mol% carboxymethylated PVIm, that is, CA1(7)-PVIm, observed in a widespread area by in vivo imaging system. These results suggest that the CA1(7)-PVIm/pDNA PIC is effective for the diffusive delivery of the pDNA into skeletal muscle for the treatment of serious muscle diseases. |
| 巻・号 | 12(15) |
| ページ | 3947-3955 |
| 公開日 | 2024-7-23 |
| DOI | 10.1039/d4bm00510d |
| PMID | 38949480 |
| MeSH | Animals DNA* / administration & dosage DNA* / chemistry Diffusion Gene Transfer Techniques Imidazoles* / administration & dosage Imidazoles* / chemistry Mice Muscle, Skeletal* / metabolism Plasmids* / administration & dosage Plasmids* / chemistry Polyvinyls* / chemistry |
| IF | 6.183 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |