Reference - Detail
| RRC ID | 83505 |
|---|---|
| Author | Miura F, Shibata Y, Miura M, Inatomi K, Suzuki Y, Ito T. |
| Title | Identification of an enzyme with strong single-stranded DNA ligation activity and its application for sequencing. |
| Journal | Nucleic Acids Res |
| Abstract |
An enzyme with strong single-stranded DNA (ssDNA) ligation activity would be advantageous for many molecular biology applications. However, currently available enzymes exhibit only limited activity. Here, we identified an enzyme with strong ssDNA ligation activity upon searching the databases for proteins homologous to TS2126 RNA ligase, the known enzyme with the highest yet limited ssDNA ligation activity. A ligase from Thermus phage phiLo (GenBank: AYJ73970) or Tph ssDNA ligase (SDL) depicted higher ssDNA ligation activity than TS2126 RNA ligase. This study suggests that SDL could be useful for various applications including sequencing for library preparation. |
| Volume | 53(3) |
| Published | 2025-1-24 |
| DOI | 10.1093/nar/gkaf054 |
| PII | 7990338 |
| PMID | 39883008 |
| PMC | PMC11780859 |
| MeSH | DNA Ligases* / metabolism DNA, Single-Stranded* / metabolism RNA Ligase (ATP) / chemistry RNA Ligase (ATP) / genetics RNA Ligase (ATP) / metabolism Sequence Analysis, DNA / methods Thermus / enzymology Thermus / genetics Viral Proteins / genetics Viral Proteins / metabolism |
| IF | 11.502 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BY20134 BY20135 BY20148 BY20149 |