論文 - 詳細
| RRC ID | 83521 |
|---|---|
| 著者 | Mizuno K, Hirashima T, Toda S. |
| タイトル | Robust tissue pattern formation by coupling morphogen signal and cell adhesion. |
| ジャーナル | EMBO Rep |
| Abstract |
Morphogens, locally produced signaling molecules, form a concentration gradient to guide tissue patterning. Tissue patterns emerge as a collaboration between morphogen diffusion and responsive cell behaviors, but the mechanisms through which diffusing morphogens define precise spatial patterns amidst biological fluctuations remain unclear. To investigate how cells respond to diffusing proteins to generate tissue patterns, we develop SYMPLE3D, a 3D culture platform. By engineering gene expression responsive to artificial morphogens, we observe that coupling morphogen signals with cadherin-based adhesion is sufficient to convert a morphogen gradient into distinct tissue domains. Morphogen-induced cadherins gather activated cells into a single domain, removing ectopically activated cells. In addition, we reveal a switch-like induction of cadherin-mediated compaction and cell mixing, homogenizing activated cells within the morphogen gradient to form a uniformly activated domain with a sharp boundary. These findings highlight the cooperation between morphogen gradients and cell adhesion in robust tissue patterning and introduce a novel method for tissue engineering to develop new tissue domains in organoids. |
| 巻・号 | 25(11) |
| ページ | 4803-4826 |
| 公開日 | 2024-11-1 |
| DOI | 10.1038/s44319-024-00261-z |
| PII | 10.1038/s44319-024-00261-z |
| PMID | 39333626 |
| PMC | PMC11549100 |
| MeSH | Animals Body Patterning / genetics Cadherins* / genetics Cadherins* / metabolism Cell Adhesion* Humans Morphogenesis Organoids / cytology Organoids / metabolism Signal Transduction* Tissue Engineering / methods |
| IF | 7.497 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 65 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB1422) |