Reference - Detail
| RRC ID | 83541 |
|---|---|
| Author | Sakamoto T. |
| Title | Live-Cell Imaging of RNA G-Quadruplex with a Dual-Color Fluorescence Switch-on Probe. |
| Journal | Methods Mol Biol |
| Abstract |
In-cell imaging of the G-quadruplex (G4) formation of nucleic acids remains challenging for revealing G4's functions in cells. This study describes the cell imaging method of G4 nucleic acids using our unique tripodal quinone-cyanine fluorescent dye, QCy(MeBT)3, whose 600 nm and 700 nm fluorescence is enhanced independently upon the binding with double-stranded DNA and G4-DNA/RNA, respectively. The use of QCy(MeBT)3 enables us to visualize cytosolic G4 RNAs in fixed and living HeLa cells with near-infrared 700 nm fluorescence. |
| Volume | 2875 |
| Pages | 83-90 |
| Published | 2025-1-1 |
| DOI | 10.1007/978-1-0716-4248-1_7 |
| PMID | 39535641 |
| MeSH | DNA / chemistry Fluorescent Dyes* / chemistry G-Quadruplexes* HeLa Cells Humans Microscopy, Fluorescence / methods Molecular Imaging / methods Optical Imaging / methods RNA* / chemistry |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |