論文 - 詳細
| RRC ID | 83572 |
|---|---|
| 著者 | Tabei Y, Nakajima Y. |
| タイトル | IL-1β-activated PI3K/AKT and MEK/ERK pathways coordinately promote induction of partial epithelial-mesenchymal transition. |
| ジャーナル | Cell Commun Signal |
| Abstract |
Epithelial-mesenchymal transition (EMT) is a cellular process in embryonic development, wound healing, organ fibrosis, and cancer metastasis. Previously, we and others have reported that proinflammatory cytokine interleukin-1β (IL-1β) induces EMT. However, the exact mechanisms, especially the signal transduction pathways, underlying IL-1β-mediated EMT are not yet completely understood. Here, we found that IL-1β stimulation leads to the partial EMT-like phenotype in human lung epithelial A549 cells, including the gain of mesenchymal marker (vimentin) and high migratory potential, without the complete loss of epithelial marker (E-cadherin). IL-1β-mediated partial EMT induction was repressed by PI3K inhibitor LY294002, indicating that the PI3K/AKT pathway plays a significant role in the induction. In addition, ERK1/2 inhibitor FR180204 markedly inhibited the IL-1β-mediated partial EMT induction, demonstrating that the MEK/ERK pathway was also involved in the induction. Furthermore, we found that the activation of the PI3K/AKT and MEK/ERK pathways occurred downstream of the epidermal growth factor receptor (EGFR) pathway and the IL-1 receptor (IL-1R) pathway, respectively. Our findings suggest that the PI3K/AKT and MEK/ERK pathways coordinately promote the IL-1β-mediated partial EMT induction. The inhibition of not one but both pathways is expected yield clinical benefits by preventing partial EMT-related disorders such as organ fibrosis and cancer metastasis. |
| 巻・号 | 22(1) |
| ページ | 392 |
| 公開日 | 2024-8-8 |
| DOI | 10.1186/s12964-024-01775-8 |
| PII | 10.1186/s12964-024-01775-8 |
| PMID | 39118068 |
| PMC | PMC11308217 |
| MeSH | A549 Cells Epithelial-Mesenchymal Transition* ErbB Receptors / metabolism Humans Interleukin-1beta* / metabolism MAP Kinase Signaling System* Phosphatidylinositol 3-Kinases* / metabolism Proto-Oncogene Proteins c-akt* / metabolism |
| IF | 4.344 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |