| RRC ID |
83580
|
| 著者 |
Su CM, Tsai CH, Chen HT, Wu YS, Yang SF, Tang CH.
|
| タイトル |
Melatonin Regulates Rheumatoid Synovial Fibroblasts-Related Inflammation: Implications for Pathological Skeletal Muscle Treatment.
|
| ジャーナル |
J Pineal Res
|
| Abstract |
Melatonin has been reported to regulate circadian rhythms and have anti-inflammatory characteristics in various inflammatory autoimmune diseases, but its effects in diseases-associated muscle atrophy remain controversial. This study is aimed to determine the evidence of melatonin in rheumatoid arthritis (RA)-related pathological muscle atrophy. We used initially bioinformatics results to show that melatonin regulated significantly the correlation between pro-inflammation and myogenesis in RA synovial fibroblasts (RASF) and myoblasts. The conditioned medium (CM) from melatonin-treated RASF was incubated in myoblasts with growth medium and differentiated medium to investigate the markers of pro-inflammation, atrophy, and myogenesis. We found that melatonin regulated RASF CM-induced pathological muscle pro-inflammation and atrophy in myoblasts and differentiated myocytes through NF-κB signaling pathways. We also showed for the first time that miR-30c-1-3p is negatively regulated by three inflammatory cytokines in human RASF, which is associated with murine-differentiated myocytes. Importantly, oral administration with melatonin in a collagen-induced arthritis (CIA) mouse model also significantly improved arthritic swelling, hind limb grip strength as well as pathological muscle atrophy. In conclusion, our study is the first to demonstrate not only the underlying mechanism whereby melatonin decreases pro-inflammation in RA-induced pathological muscle atrophy but also increases myogenesis in myoblasts and differentiated myocytes.
|
| 巻・号 |
76(6)
|
| ページ |
e13009
|
| 公開日 |
2024-9-1
|
| DOI |
10.1111/jpi.13009
|
| PMID |
39315577
|
| MeSH |
Animals
Arthritis, Experimental / drug therapy
Arthritis, Experimental / metabolism
Arthritis, Experimental / pathology
Arthritis, Rheumatoid* / drug therapy
Arthritis, Rheumatoid* / metabolism
Arthritis, Rheumatoid* / pathology
Fibroblasts* / drug effects
Fibroblasts* / metabolism
Fibroblasts* / pathology
Humans
Inflammation / metabolism
Inflammation / pathology
Male
Melatonin* / pharmacology
Mice
Mice, Inbred DBA
Muscle, Skeletal* / drug effects
Muscle, Skeletal* / metabolism
Muscle, Skeletal* / pathology
Muscular Atrophy / drug therapy
Muscular Atrophy / metabolism
Muscular Atrophy / pathology
Myoblasts / drug effects
Myoblasts / metabolism
Synovial Membrane / drug effects
Synovial Membrane / metabolism
Synovial Membrane / pathology
|
| IF |
14.528
|
| リソース情報 |
| ヒト・動物細胞 |
MH7A(RCB1512) |
