論文 - 詳細
| RRC ID | 83585 |
|---|---|
| 著者 | Muneshige K, Hatakeyama R. |
| タイトル | Vacuoles provide the source membrane for TORC1-containing signaling endosomes. |
| ジャーナル | J Cell Biol |
| Abstract |
Organelle biogenesis is fundamental to eukaryotic cell biology. Yeast signaling endosomes were recently identified as a signaling platform for the evolutionarily conserved Target of Rapamycin Complex 1 (TORC1) kinase complex. Despite the importance of signaling endosomes for TORC1-mediated control of cellular metabolism, how this organelle is generated has been a mystery. Here, we developed a system to induce synchronized de novo formation of signaling endosomes, enabling real-time monitoring of their biogenesis. Using this system, we identify vacuoles as a membrane source for newly formed signaling endosomes. Membrane supply from vacuoles is mediated by the CROP membrane-cutting complex, consisting of Atg18 PROPPIN and retromer subunits. The formation of signaling endosomes requires TORC1 activity, suggestive of a tightly regulated process. This study unveiled the first mechanistic principles and molecular participants of signaling endosome biogenesis. |
| 巻・号 | 224(5) |
| 公開日 | 2025-5-5 |
| DOI | 10.1083/jcb.202407021 |
| PII | 277308 |
| PMID | 40052923 |
| PMC | PMC11893502 |
| MeSH | Endosomes* / metabolism Intracellular Membranes / metabolism Mechanistic Target of Rapamycin Complex 1 / genetics Mechanistic Target of Rapamycin Complex 1 / metabolism Saccharomyces cerevisiae* / genetics Saccharomyces cerevisiae* / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Signal Transduction* Transcription Factors Vacuoles* / metabolism |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP9806 |