Reference - Detail
| RRC ID | 83599 |
|---|---|
| Author | Yoshino H, Tadano K, Omiya C, Ishii K, Munakata N. |
| Title | Involvement of cellular senescence in the effect of X-irradiated human lung fibroblast WI-38 cells on human lung cancer A549 cell clonogenic potential. |
| Journal | Radiat Prot Dosimetry |
| Abstract |
Ionizing radiation not only affects irradiated but also non-irradiated surrounding cells through intercellular communication, indicating that the former cells could affect the latter. The present study investigated the effect of X-irradiated normal human lung fibroblast WI-38 cells on the clonofenic potential of human lung cancer A549 cells by co-culturing them. Moreover, the relationship between the effects of co-culturing on the clonogenic potential of A549 cells and cellular senescence in WI-38 cells was investigated. The co-culture with 10-Gy-irradiated WI-38 cells and A549 cells enhanced the clonogenic potential of non- or X-irradiated A549 cells. Irradiated WI-38 cells exhibited high SA-β-gal activity, a cellular senescence hallmark. Importantly, treatment with senolytic drugs, which eliminate senescent cells, not only influenced high-SA-β-gal-activity cell percentages among the irradiated WI-38 cells but also the effect of irradiated WI-38 cells on the clonogenic potential of A549 cells. In conclusion, our results suggest that irradiated WI-38 cells promote A549 cell clonogenic potential and irradiated senescent WI-38 cells contribute to this effect. |
| Volume | 200(16-18) |
| Pages | 1608-1614 |
| Published | 2024-11-13 |
| DOI | 10.1093/rpd/ncae089 |
| PII | 7899477 |
| PMID | 39540500 |
| MeSH | A549 Cells Cellular Senescence* / radiation effects Coculture Techniques Fibroblasts* / radiation effects Humans Lung / cytology Lung / radiation effects Lung Neoplasms* / pathology Lung Neoplasms* / radiotherapy X-Rays |
| IF | 0.773 |
| Altmetric score |
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | WI-38 A549 |