Reference - Detail
| RRC ID | 83627 |
|---|---|
| Author | Zhang H, Fang J, Liu Y, Zhu W, Xu Y, Zhang Y, Shen W, Li D, Hao C. |
| Title | Hsa_circ_0043532 contributes to PCOS through upregulation of CYP19A1 by acting as a ceRNA for hsa-miR-1270. |
| Journal | J Ovarian Res |
| Abstract |
BACKGROUND:Polycystic ovarian syndrome (PCOS) accounts for about 75% of anovulatory infertility. The cause of PCOS is not clear. CircRNAs acting as miRNA sponges mediate the post-transcriptional regulation of multiple genes. CYP19A1 is a limiting enzyme in the ovarian steroidogenesis pathway. However, the mechanism of circRNAs regulating granulosa cell (GC) estradiol secretion in PCOS remains to be elucidated. METHODS:Bioinformatics was used to predict the potential target miRNAs of circ_0043532 and target genes of miR-1270. Target miRNAs and mRNA expression were verified by qRT-PCR in GCs from 45 women with PCOS and 65 non-PCOS. Western blot, ELISA and dual-luciferase reporter assays were applied to confirm the substrate of miR-1270. RESULTS:Circ_0043532 and CYP19A1 were significant up-regulation in GCs from patients with PCOS. The predicted target miRNAs of circ_0053432, miR-1270, miR-576-5p, miR-421 and miR-142-5p, were notably decreased in GCs from patients with PCOS. Mechanistic experiments showed that circ_0043532 specifically binds to miR-1270. MiR-1270 was negatively regulated by circ_0043532. Concomitantly, miR-1270 inhibited CYP19A1 expression and estradiol production, which could be reversed by circ_0043532 over-expression. CONCLUSION:We identified that circ_0043532/miR-1270/CYP19A1 axis contributes to the aberrant steroidogenesis of GCs from patients with PCOS. This study broadens the spectrum of pathogenic factors of PCOS, and circ_0043532 might be a potential therapeutic target for PCOS. |
| Volume | 17(1) |
| Pages | 151 |
| Published | 2024-7-22 |
| DOI | 10.1186/s13048-024-01474-5 |
| PII | 10.1186/s13048-024-01474-5 |
| PMID | 39039600 |
| PMC | PMC11265019 |
| MeSH | Adult Aromatase* / genetics Aromatase* / metabolism Female Granulosa Cells / metabolism Humans MicroRNAs* / genetics MicroRNAs* / metabolism Polycystic Ovary Syndrome* / genetics Polycystic Ovary Syndrome* / metabolism RNA, Circular* / genetics RNA, Circular* / metabolism RNA, Competitive Endogenous Up-Regulation* |
| IF | 2.469 |
| Resource | |
| Human and Animal Cells | KGN(RCB1154) |