論文 - 詳細
| RRC ID | 83706 |
|---|---|
| 著者 | Milano SN, Bayer LV, Ko JJ, Casella CE, Bratu DP. |
| タイトル | The role of ER exit sites in maintaining P-body organization and integrity during Drosophila melanogaster oogenesis. |
| ジャーナル | EMBO Rep |
| Abstract |
Processing bodies (P-bodies) are cytoplasmic membrane-less organelles which host multiple mRNA processing events. While the fundamental principles of P-body organization are beginning to be elucidated in vitro, a nuanced understanding of how their assembly is regulated in vivo remains elusive. Here, we investigate the potential link between ER exit sites and P-bodies in Drosophila melanogaster egg chambers. Employing a combination of live and super-resolution imaging, we find that P-bodies associated with ER exit sites are larger and less mobile than cytoplasmic P-bodies, indicating that they constitute a distinct class of P-bodies. Moreover, we demonstrate that altering the composition of ER exit sites has differential effects on core P-body proteins (Me31B, Cup, and Trailer Hitch), suggesting a potential role for ER exit sites in P-body organization. Furthermore, we show that in the absence of ER exit sites, P-body integrity is compromised and the stability and translational repression efficiency of the maternal mRNA, oskar, are reduced. Together, our data highlights the crucial role of ER exit sites in governing P-body organization. |
| 巻・号 | 26(2) |
| ページ | 494-520 |
| 公開日 | 2025-1-1 |
| DOI | 10.1038/s44319-024-00344-x |
| PII | 10.1038/s44319-024-00344-x |
| PMID | 39653851 |
| PMC | PMC11772875 |
| MeSH | Animals DEAD-box RNA Helicases Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Endoplasmic Reticulum* / metabolism Female Oogenesis* RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 7.497 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#115161 |