論文 - 詳細
| RRC ID | 83733 |
|---|---|
| 著者 | Chavan A, Skrutl L, Uliana F, Pfister M, Brändle F, Tirian L, Baptista D, Handler D, Burke D, Sintsova A, Beltrao P, Brennecke J, Jagannathan M. |
| タイトル | Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression. |
| ジャーナル | PLoS Biol |
| Abstract |
Noncoding satellite DNA repeats are abundant at the pericentromeric heterochromatin of eukaryotic chromosomes. During interphase, sequence-specific DNA-binding proteins cluster these repeats from multiple chromosomes into nuclear foci known as chromocenters. Despite the pivotal role of chromocenters in cellular processes like genome encapsulation and gene repression, the associated proteins remain incompletely characterized. Here, we use 2 satellite DNA-binding proteins, D1 and Prod, as baits to characterize the chromocenter-associated proteome in Drosophila embryos, ovaries, and testes through quantitative mass spectrometry. We identify D1- and Prod-associated proteins, including known heterochromatin proteins as well as proteins previously unlinked to satellite DNA or chromocenters, thereby laying the foundation for a comprehensive understanding of cellular functions enabled by satellite DNA repeats and their associated proteins. Interestingly, we find that multiple components of the transposon-silencing piRNA pathway are associated with D1 and Prod in embryos. Using genetics, transcriptomics, and small RNA profiling, we show that flies lacking D1 during embryogenesis exhibit transposon expression and gonadal atrophy as adults. We further demonstrate that this gonadal atrophy can be rescued by mutating the checkpoint kinase, Chk2, which mediates germ cell arrest in response to transposon mobilization. Thus, we reveal that a satellite DNA-binding protein functions during embryogenesis to silence transposons, in a manner that is heritable across later stages of development. |
| 巻・号 | 23(1) |
| ページ | e3002984 |
| 公開日 | 2025-1-1 |
| DOI | 10.1371/journal.pbio.3002984 |
| PII | PBIOLOGY-D-24-02660 |
| PMID | 39813297 |
| PMC | PMC11734925 |
| MeSH | Animals DNA Transposable Elements* / genetics DNA, Satellite* / genetics DNA, Satellite* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / embryology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Female Heterochromatin* / genetics Heterochromatin* / metabolism Male Ovary / metabolism Proteome* / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Testis / metabolism |
| IF | 7.076 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.8 |
| リソース情報 | |
| ショウジョウバエ | DGRC#111248 |