論文 - 詳細
| RRC ID | 83755 |
|---|---|
| 著者 | Yamashita K, Shimane K, Muramoto T. |
| タイトル | Optogenetic control of cAMP oscillations reveals frequency-selective transcription factor dynamics in Dictyostelium. |
| ジャーナル | Development |
| Abstract |
Oscillatory dynamics and their modulation are crucial for cellular decision-making; however, analysing these dynamics remains challenging. Here, we present a tool that combines the light-activated adenylate cyclase mPAC with the cAMP biosensor Pink Flamindo, enabling precise manipulation and real-time monitoring of cAMP oscillation frequencies in Dictyostelium. High-frequency modulation of cAMP oscillations induced cell aggregation and multicellular formation, even at low cell densities, such as a few dozen cells. At the population level, chemotactic aggregation is driven by modulated frequency signals. Additionally, modulation of cAMP frequency significantly reduced the amplitude of the shuttling behaviour of the transcription factor GtaC, demonstrating low-pass filter characteristics capable of converting subtle oscillation changes, such as from 6 min to 4 min, into gene expression. These findings enhance our understanding of frequency-selective cellular decoding and its role in cellular signalling and development. |
| 巻・号 | 152(1) |
| 公開日 | 2025-1-1 |
| DOI | 10.1242/dev.204403 |
| PII | 364955 |
| PMID | 39775856 |
| PMC | PMC11829771 |
| MeSH | Adenylyl Cyclases / metabolism Biosensing Techniques Chemotaxis Cyclic AMP* / metabolism Dictyostelium* / genetics Dictyostelium* / metabolism Optogenetics* / methods Protozoan Proteins / genetics Protozoan Proteins / metabolism Signal Transduction Transcription Factors* / genetics Transcription Factors* / metabolism |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | Dictyostelium AX3 |